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biotinylated vicia villosa lectin (vvl, vva)  (Vector Laboratories)


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    Vector Laboratories biotinylated vicia villosa lectin (vvl, vva)
    Biotinylated Vicia Villosa Lectin (Vvl, Vva), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+vva+lectin/Biotinylated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/custom%40b-1235%4042414656
    Average 95 stars, based on 164 article reviews
    biotinylated vicia villosa lectin (vvl, vva) - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription
    Article Snippet: Proteins were transferred to a polyvinylidene di uoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4°C and then it was incubated for 1 h at 37°C with HRP-conjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription
    Article Snippet: Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4 °C and then it was incubated for 1 h at 37 °C with HRP-conjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription.
    Article Snippet: Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4 °C and then it was incubated for 1 h at 37 °C with HRPconjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Incubation:

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription
    Article Snippet: Proteins were transferred to a polyvinylidene di uoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4°C and then it was incubated for 1 h at 37°C with HRP-conjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Article Title: A multivalent CD44 glycoconjugate vaccine candidate for cancer immunotherapy.
    Article Snippet: Cancer presents a high mortality rate due to ineffective treatments and tumour relapse with progression.. Cancer vaccines hold tremendous potential due to their capability to eradicate tumour and prevent relapse.. In this study, we present a novel glycovaccine for precise targeting and immunotherapy of aggressive solid tumours that overexpress CD44 standard isoform (CD44s) carrying immature Tn and sialyl-Tn (sTn) O-glycans.

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription
    Article Snippet: Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4 °C and then it was incubated for 1 h at 37 °C with HRP-conjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Article Title: CCDC88C, an O-GalNAc glycosylation substrate of GALNT6, drives breast cancer metastasis by promoting c-JUN-mediated CEMIP transcription.
    Article Snippet: Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, China). .. The membrane was incubated with primary antibodies or biotinylated VVA lectin (Cat#B-1235, Vector Laboratories, USA) overnight at 4 °C and then it was incubated for 1 h at 37 °C with HRPconjugated secondary antibodies for or HRP-conjugated streptavidin (Cat#SA00001-0, Proteintech, USA) for biotin detection. .. Finally, the membrane was developed with ECL western blotting substrate (Solarbio, China).

    Blocking Assay:

    Article Title: A multivalent CD44 glycoconjugate vaccine candidate for cancer immunotherapy.
    Article Snippet: Cancer presents a high mortality rate due to ineffective treatments and tumour relapse with progression.. Cancer vaccines hold tremendous potential due to their capability to eradicate tumour and prevent relapse.. In this study, we present a novel glycovaccine for precise targeting and immunotherapy of aggressive solid tumours that overexpress CD44 standard isoform (CD44s) carrying immature Tn and sialyl-Tn (sTn) O-glycans.

    Article Title: Chronic silencing of subsets of cortical layer 5 pyramidal neurons has a long‐term influence on the laminar distribution of parvalbumin interneurons and the perineuronal nets
    Article Snippet: .. Rabbit anti‐PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B‐1235‐2, Vector Laboratories, Burlingame, US) was used at 2 μg/mL concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton‐X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato‐expressing axonal processes and Vglut1‐positive synapses colocalise in the striatum, slices were incubated with guinea pig anti‐Vglut1 (1:500, EMD Millipore AB5905, lot: 3258729).

    Article Title: Chronic abolition of evoked vesicle release from layer 5 projection neurons disrupts the laminar distribution of parvalbumin interneurons in the adult cortex
    Article Snippet: .. Rabbit anti-PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland ) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B-1235-2, Vector Laboratories, Burlingame, US) was used at 2 μg/ml concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton-X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato-expressing axonal processes and VGLUT1-positive synapses colocalize in the striatum, slices were incubated with guinea pig anti-VGLUT1 (1:500, EMD Millipore AB5905, lot: 3258729).

    Article Title: Chronic silencing of subsets of cortical layer 5 pyramidal neurons has a long-term influence on the laminar distribution of parvalbumin interneurons and the perineuronal nets.
    Article Snippet: .. Rabbit anti- PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B- 1235- 2, Vector Laboratories, Burlingame, US) was used at 2 μg/mL concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton- X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato- expressing axonal processes and Vglut1- positive synapses colocalise in the striatum, slices were incubated with guinea pig anti- Vglut1 (1:500, EMD Millipore AB5905, lot: 3258729).

    Concentration Assay:

    Article Title: Chronic silencing of subsets of cortical layer 5 pyramidal neurons has a long‐term influence on the laminar distribution of parvalbumin interneurons and the perineuronal nets
    Article Snippet: .. Rabbit anti‐PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B‐1235‐2, Vector Laboratories, Burlingame, US) was used at 2 μg/mL concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton‐X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato‐expressing axonal processes and Vglut1‐positive synapses colocalise in the striatum, slices were incubated with guinea pig anti‐Vglut1 (1:500, EMD Millipore AB5905, lot: 3258729).

    Article Title: Chronic abolition of evoked vesicle release from layer 5 projection neurons disrupts the laminar distribution of parvalbumin interneurons in the adult cortex
    Article Snippet: .. Rabbit anti-PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland ) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B-1235-2, Vector Laboratories, Burlingame, US) was used at 2 μg/ml concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton-X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato-expressing axonal processes and VGLUT1-positive synapses colocalize in the striatum, slices were incubated with guinea pig anti-VGLUT1 (1:500, EMD Millipore AB5905, lot: 3258729).

    Article Title: Chronic silencing of subsets of cortical layer 5 pyramidal neurons has a long-term influence on the laminar distribution of parvalbumin interneurons and the perineuronal nets.
    Article Snippet: .. Rabbit anti- PV (Cat# PV27, RRID:AB_2631173, Swant, Marly, Switzerland) was diluted at 1:5000 for postnatal and 1:500 for adult brains, and the biotinylated VVA lectin (B- 1235- 2, Vector Laboratories, Burlingame, US) was used at 2 μg/mL concentration and diluted in a blocking solution consisting of 2% donkey serum and 0.2% Triton- X100 diluted in VVA buffer. .. To ascertain whether Rbp4/tdTomato- expressing axonal processes and Vglut1- positive synapses colocalise in the striatum, slices were incubated with guinea pig anti- Vglut1 (1:500, EMD Millipore AB5905, lot: 3258729).



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